Here, 30 substrates and probability of positive reactions to the substrates have been tested for spore-formers to make the computer software for final identification. The bacteria cultured overnight are to be inoculated in the Kit and cultured for 18-24 hr at 35-37 deg C with the lid attached by substrates for identification. Isolation and identification of a spore-forming bacterium have to be confirmed by phase-contrast microscopy. The part III in this title series describes the development of software for identification of spore-forming bacteria using the commercially available BBL CRYSTAL GP Identification Kit (Becton, Dickinson and Co., Ltd.), which is essentially for identification of Gram positive bacteria and is not always suitable for the spore-former in the radiation sterilization of medical devices. Hironiwa, Takayuki Yamamoto, Yoko Koshikawa, Tomihiko International Nuclear Information System (INIS) Development of identification software of spore-forming bacteria by using BBL CRYSTAL GP identification kit Microbiological method for radiation sterilization (III). The pathway is closely related to the d-tagatose pathway of the Gram-negative bacterium Klebsiella oxytoca, in contrast to the d-tagatose 6-phosphate pathway described in the Gram-positive bacterium Staphylococcus aureus. We report the first identification of a gene cluster involved in d-tagatose catabolism in Bacillus licheniformis. Van der Heiden, Edwige Lebrun, Sarah Freichels, Régine Brans, Alain Vastenavond, Christian M. Van der Heiden, Edwige Delmarcelle, Michaël Lebrun, Sarah Freichels, Régine Brans, Alain Vastenavond, Christian M. The pathway is closely related to the d-tagatose pathway of the Gram-negative bacterium Klebsiella oxytoca, in contrast to the d-tagatose 6-phosphate pathway described in the Gram-positive bacterium Staphylococcus aureus.Ī Pathway Closely Related to the d-Tagatose Pathway of Gram-Negative Enterobacteria Identified in the Gram-Positive Bacterium Bacillus licheniformis Van der Heiden, Edwige Delmarcelle, Michaël Lebrun, Sarah Freichels, Régine Brans, Alain Vastenavond, Christian M Galleni, Moreno Joris, Bernard is proposed.Ī pathway closely related to the (D)-tagatose pathway of gram-negative enterobacteria identified in the gram-positive bacterium Bacillus licheniformis. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, strain P9(T) (=DSM 23228(T)=LMG 25523(T)) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. These two recognized species formed a coherent cluster with strain P9(T) that was supported by a bootstrap value of 99%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P9(T) was most closely related to Jeotgalibacillus campisalis DSM 18983(T) (96.8%) and Jeotgalibacillus marinus DSM 1297(T) (96.5%). The genomic DNA G+C content was about 39.4 mol%. The cell-wall peptidoglycan was of the A1α type, with L-lysine as the diagnostic diamino acid. It had an optimum growth temperature of about 35 Â☌ and an optimum growth pH of about 8.0-8.5, and grew in medium with 0-9% (w/v) NaCl. This organism was aerobic and catalase- and oxidase- positive. nov., a Gram-stain-positive bacterium isolated from soil.Ĭunha, Sofia Tiago, Igor Paiva, Gabriel Nobre, Fernanda da Costa, Milton S VerÃssimo, AntónioĪ Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9(T), was isolated from soil in Portugal. Research on the exosporium is proposed as a path toward improved means of sterilization, medical treatment, and prevention of biofouling. The exosporium has been conjectured to play a role in the elevated resistance to sterilization. odysseensis is a Gram-positive, facultatively anaerobic, rod-shaped bacterium that forms round spores. odysseensis because it was isolated from surfaces of the Mars Odyssey spacecraft as part of continuing research on techniques for sterilizing spacecraft to prevent contamination of remote planets by terrestrial species. Spore-Forming Bacteria that Resist SterilizationĪ report presents a phenotypic and genotypic characterization of a bacterial species that has been found to be of the genus Bacillus and has been tentatively named B.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |